Services for standard mouse strain maintenance
- Rederivation of mouse strains from cryoconserved sperm and embryos, incl. IVF
- Sperm and embryo cryoconservation, optionally with rederivation test
- Embryo transfer into foster animals for strain cleanup
Established transgenic services
- Design and consulting on transgenes and strategies
- Generation of complex targeting constructs with next generation conditional knock-out strategies
- Assistance for license application from the authorities for novel mouse lines
- Microinjection of nucleic acids and riboprotein CAS9 complexes into zygotes (pronucleus or cytoplasm)
- Ex vivo zygote electroporation with riboprotein CAS9 complexes
- ES cells gene targeting with screening for clonal selection
- ES cell aggregation with diploid/tetraploid host cells for embryo and strain generation
- ES cell microinjection of blastocysts
Future transgenic services
(establishing phase)
- i-GONAD, in utero electroporation for knock-out and small knock-in approaches