Forschen für das Leben
Georg-Speyer-Haus. Foto: Andreas Reeg, Tel: +40-171-5449247, andreas.reeg@t-online.de, www.andreasreeg.de
Georg-Speyer-Haus. Foto: Andreas Reeg, Tel: +40-171-5449247, andreas.reeg@t-online.de, www.andreasreeg.de
Georg-Speyer-Haus. Foto: Andreas Reeg, Tel: +40-171-5449247, andreas.reeg@t-online.de, www.andreasreeg.de
Georg-Speyer-Haus. Foto: Andreas Reeg, Tel: +40-171-5449247, andreas.reeg@t-online.de, www.andreasreeg.de

Dr. Phillip Grote

gsh-pgrote-2021

Head of TCF

T: +4969-63395-620
Email: Ph.Grote@georg-speyer-haus.de

Services for standard mouse strain maintenance

 

  • Rederivation of mouse strains from cryoconserved sperm and embryos, incl. IVF
  • Sperm and embryo cryoconservation, optionally with rederivation test
  • Embryo transfer into foster animals for strain cleanup

 

 

Established transgenic services

 

  • Design and consulting on transgenes and strategies
  • Generation of complex targeting constructs with next generation conditional knock-out strategies
  • Assistance for license application from the authorities for novel mouse lines
  • Microinjection of nucleic acids and riboprotein CAS9 complexes into zygotes (pronucleus or cytoplasm)
  • Ex vivo zygote electroporation with riboprotein CAS9 complexes
  • ES cells gene targeting with screening for clonal selection
  • ES cell aggregation with diploid/tetraploid host cells for embryo and strain generation
  • ES cell microinjection of blastocysts

 

Future transgenic services

(establishing phase)

  • i-GONAD, in utero electroporation for knock-out and small knock-in approaches

 

Contact via tcf@georg-speyer-haus.de